David Functional Annotation Site

If you’ve just finished an RNA-seq or microarray experiment, you probably have a list of genes. It might be a short list of 50 names or a long one of 2,000. But a list is just data. The real question is: What does this list mean biologically?

Your brain cannot synthesize that noise. DAVID can.

Go to [david.ncifcrf.gov] and turn your data into discovery. Have a favorite alternative (Enrichr, g:Profiler, Metascape)? Drop a comment below. But for my money, DAVID is still the gold standard for functional annotation. david functional annotation

Enter (The Database for Annotation, Visualization and Integrated Discovery). For nearly two decades, DAVID has been the Swiss Army knife of functional annotation. It answers the golden question of genomics: "Which biological processes are my genes involved in?"

But DAVID is

This is the most critical step users mess up. You must tell DAVID what the "universe" is. Are you looking at the whole human genome? Or just the 10,000 genes expressed in your specific tissue? Use the whole genome for standard enrichment, but use a tissue-specific background for precision.

For 80% of biologists who need to answer, "What is my gene list doing?" DAVID is the best tool ever made. Use it, cite it, and never present a raw gene list to your PI again. If you’ve just finished an RNA-seq or microarray

DAVID uses . Instead of reading genes, it reads Gene Ontology (GO) terms, pathways (KEGG), protein domains (InterPro), and disease associations. How DAVID Works (The 3-Step Magic) Step 1: Upload your list. Paste your gene symbols, Entrez IDs, or Affymetrix probes. You don't need to know the format; DAVID auto-detects it.